Sonic Hedgehog antibody (N-Term)

Catalog No. ABIN3032696

The Rabbit Polyclonal anti-Sonic Hedgehog antibody has been validated for WB and ELISA. It is suitable to detect Sonic Hedgehog in samples from Mouse and Rat.

Quick Overview for Sonic Hedgehog antibody (N-Term) (ABIN3032696)

Target: Sonic Hedgehog (SHH)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: N-Term

Purification: Antigen affinity purified

Immunogen: This mouse Shh antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 58-91 amino acids from the N-terminal region of mouse Sonic hedgehog.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Shh antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The 45 kDa precursor protein autocleaves into 27 kDa amino and 19 kDa carboxy fragments.\. Western blot: 1:1000-2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Shh antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Shh

Background: Sonic hedgehog intercellular signaling is essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. [UniProt]

UniProt: Q62226

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development