SMAD4 antibody (AA 400-428)

Catalog No. ABIN3032608

This Rabbit Polyclonal antibody specifically detects SMAD4 in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for SMAD4 antibody (AA 400-428) (ABIN3032608)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SMAD4 Antibody

Binding Specificity: AA 400-428

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Pig

Purification: Purified

Immunogen: A portion of amino acids 400-428 from the human protein was used as the immunogen for this SMAD4 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SMAD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background: In muscle physiology, SMAD4 plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). [UniProt]

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy