SPHK2 antibody (AA 590-620)

Catalog No. ABIN3032713

This Rabbit Polyclonal antibody specifically detects SPHK2 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for SPHK2 antibody (AA 590-620) (ABIN3032713)

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-SPHK2 Antibody

Binding Specificity: AA 590-620

Purification: Purified

Immunogen: A portion of amino acids 590-620 from the human protein was used as the immunogen for this SPHK2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the SPHK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SPHK2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SPHK2

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Alternative Name: SPHK2

Background: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

UniProt: Q9NRA0

Pathways: VEGF Signaling