Src antibody (AA 24-52)

Catalog No. ABIN3029554

This Rabbit Polyclonal antibody specifically detects Src in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Src antibody (AA 24-52) (ABIN3029554)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Src antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Src Antibody

Binding Specificity: AA 24-52

Cross-Reactivity (Details): Expected species reactivity: Mouse

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 24-52 from the human protein was used as the immunogen for this Src antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:2000,IHC (Paraffin): 1:50,Flow Cytometry: 1:50,Immunofluorescence: 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: Src

Background: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling