TBP antibody (AA 210-239)

Catalog No. ABIN3029099

The Rabbit Polyclonal anti-TBP antibody has been validated for WB, ELISA, IHC, IF and FACS. It is suitable to detect TBP in samples from Human.

Quick Overview for TBP antibody (AA 210-239) (ABIN3029099)

Target: TBP (TATA Box Binding Protein (TBP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TBP Antibody

Binding Specificity: AA 210-239

Cross-Reactivity (Details): Expected species reactivity: Bovine, Chicken, Mouse, Primate, Xenopus, Zebrafish

Purification: Purified

Immunogen: A portion of amino acids 210-239 from the human protein was used as the immunogen for this TBP antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the TBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the TBP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TBP

Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.

UniProt: P20226

Pathways: WNT Signaling