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Vimentin antibody (AA 63-90)

Name: Vimentin antibody (AA 63-90)
SKU: ABIN3029590
Price: 649.42 USD
Availability: InStock

This Rabbit Polyclonal antibody specifically detects Vimentin in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Western blot analysis of Vimentin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2). (Vimentin antibody (AA 63-90))

Fluorescent confocal image of SY5Y cells stained with Vimentin antibody at 1:200. (Vimentin antibody (AA 63-90))

Western blot analysis of Vimentinin antibody and HeLa lysate (Vimentin antibody (AA 63-90))

IHC analysis of FFPE human breast carcinoma tissue stained with Vimentin antibody (Vimentin antibody (AA 63-90))

IHC

6 images

Catalog No. ABIN3029590

Quick Overview for Vimentin antibody (AA 63-90) (ABIN3029590)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Reactivity

Human, Mouse

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Discover our top product VIM Primary Antibody

Binding Specificity
34

32

17

16

12

10

9

7

5

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 63-90

Cross-Reactivity (Details)

Expected species reactivity: Bovine, Hamster, Primate, Rat

Purification

Antigen affinity purified

Immunogen

A portion of amino acids 63-90 from the human protein was used as the immunogen for this Vimentin antibody.

Isotype

Ig Fraction
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated

Application Notes

Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the Vimentin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

Vimentin (VIM)

Alternative Name

Vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis