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Vimentin antibody

The Rabbit Polyclonal anti-Vimentin antibody has been validated for WB, IHC, ELISA, IF and FACS. It is suitable to detect Vimentin in samples from Human.
Catalog No. ABIN3029591

Quick Overview for Vimentin antibody (ABIN3029591)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Vimentin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
  • Purification

    Antigen affinity purified

    Immunogen

    This Vimentin antibody was produced from rabbits immunized with human Vimentin recombinant protein.

    Isotype

    Ig Fraction
  • Application Notes

    Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:2000,IHC (Paraffin): 1:50,Flow Cytometry: 1:50,Immunofluorescence: 1:50

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    In 1X PBS pH 7.4 with 0.09 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Aliquot the Vimentin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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