GAPDH antibody (C-Term)

Catalog No. ABIN3031089

This anti-GAPDH antibody is a Rabbit Polyclonal antibody detecting GAPDH in WB and ELISA. Suitable for Human and Zebrafish (Danio rerio).

Quick Overview for GAPDH antibody (C-Term) (ABIN3031089)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GAPDH Antibody

Binding Specificity: C-Term

Purification: Antigen affinity purified

Immunogen: This Gapdh antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 273-298 amino acids from the C-terminal region of zebrafish Gapdh.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Zebrafish Gapdh antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Zebrafish Gapdh antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins (By similarity).

UniProt: Q5XJ10