Actin antibody

Catalog No. ABIN4966073

The Mouse Monoclonal anti-Actin antibody is suitable to detect Actin in samples from Arabidopsis thaliana and Dictyostelium sp.. It has been validated for WB and IHC.

Quick Overview for Actin antibody (ABIN4966073)

Target: Actin

Reactivity: Arabidopsis thaliana, Dictyostelium sp.

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: MAbGPa 10-B3

Product Details anti-Actin Antibody

Purpose: Anti-ACT | Actin (monoclonal, clone mAbGPa 10-B3)

Cross-Reactivity (Details): Arabidopsis thaliana, Dictyostelium discoideum
Not reactive in: No confirmed exceptions from predicted reactivity are currently known.

Predicted Reactivity: Actinidia deliciosa,Brachypodium sylvaticum,Brassica napus,Brassica oleracea,Camelina sativa,Camellia lipoensis,Cucumis sativus,Dendrocalamus latiflorus,Dionaea muscipula,Gossypium sp.,Eucalyptus grandis,Euphorbia lathyris,Gynura bicolor,Ficus microcarpa,Haloxylon ammodendron,Helianthus annuus,Hevea brasiliensis,Lilium regale,Medicago sativa,Mimosa pudica,Nitraria sibirica,Oryza sativa subsp. japonica,Oryza sativa subsp. indica,Oxytropis ochrocephala,Paeonia lactiflora,Panax notoginseng,Populus trichocarpa,Prunus avium,Ricinus communis,Striga asiatica,Theobroma cacao,Torenia fournieri,Trifolium pRatense,Ziziphus jujuba

Purification: Protein G purified

Immunogen: Full-length recombinant Arabidopsis thaliana Actin-8 (ACT8) expressed in E.coli

Isotype: IgG2b

Application Details

Application Notes: 1-2 μg/mL (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunoglobulin IgG2b, Protein G purified in 0.1 M Sodium Phosphate, pH 7.4. Contains 0.15 M NaCl, 0.05 % (w/v) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for Actin

Target: Actin

Alternative Name: Actin (ACT)

Background:

Background: Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

Additional information: This antibody is purified on a protein G column. It recognizes Arabidopsis actins ACT1, 2, 3, 4, 7, 8, 11, 12 and Dictyostelium actin

Molecular Weight: 45 | 45 kDa

UniProt: Q96293