AGO10 antibody
This anti-AGO10 antibody is a Rabbit Polyclonal antibody detecting AGO10 in WB. Suitable for Arabidopsis thaliana.
Catalog No. ABIN4966095
Quick Overview for AGO10 antibody (ABIN4966095)
Target
AGO10 (ZLL)
(eIF2C Family Protein Argonaute 10 (ZLL))
Reactivity
Arabidopsis thaliana
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Cross-Reactivity (Details)
- Not reactive in: Zea mays
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Predicted Reactivity
- A. lyrata, B. napus, C. rubella, C. clementina, C. sinensis, E. salsugineum, G. arboreum, G. raimondii
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Immunogen
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO10 protein sequence, Uniprot: Q9XGW1, TAIR: AT5G43810
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Application Notes
- 1: 10 000 (WB)
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Comment
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AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
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Restrictions
- For Research Use only
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Reconstitution
- For reconstitution add 50 μL of sterile water.
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- AGO10 (ZLL) (eIF2C Family Protein Argonaute 10 (ZLL))
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Alternative Name
- Argonaute 10 (AGO10)
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Background
- AGO10 is involved in miRNA binding, and in RNA-mediated posttranscriptional gene silencing (PTGS).
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Molecular Weight
- 110.9 kDa
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UniProt
- Q9XGW1
Target
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