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AGO10 antibody

ZLL Reactivity: Arabidopsis thaliana WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN4966095
  • Target
    AGO10 (ZLL) (eIF2C Family Protein Argonaute 10 (ZLL))
    Reactivity
    Arabidopsis thaliana
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB)
    Cross-Reactivity (Details)
    Not reactive in: Zea mays
    Predicted Reactivity
    A. lyrata, B. napus, C. rubella, C. clementina, C. sinensis, E. salsugineum, G. arboreum, G. raimondii
    Immunogen
    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO10 protein sequence, Uniprot: Q9XGW1, TAIR: AT5G43810
  • Application Notes
    1: 10 000 (WB)
    Comment

    AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

    Restrictions
    For Research Use only
  • Reconstitution
    For reconstitution add 50 μL of sterile water.
    Storage Comment
    Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target
    AGO10 (ZLL) (eIF2C Family Protein Argonaute 10 (ZLL))
    Alternative Name
    Argonaute 10 (AGO10)
    Synonyms
    AGO10 antibody, ARGONAUTE 10 antibody, ARGONAUTE PROTEIN 10 antibody, MQD19.17 antibody, MQD19_17 antibody, PINHEAD antibody, PNH antibody, ZWILLE antibody, Stabilizer of iron transporter SufD / Polynucleotidyl transferase antibody, AGO10 antibody
    Background
    AGO10 is involved in miRNA binding, and in RNA-mediated posttranscriptional gene silencing (PTGS).
    Molecular Weight
    110.9 kDa
    UniProt
    Q9XGW1
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