NBR1 antibody

Catalog No. ABIN4966109

The Rabbit Polyclonal anti-NBR1 antibody has been validated for WB. It is suitable to detect NBR1 in samples from Arabidopsis thaliana.

Quick Overview for NBR1 antibody (ABIN4966109)

Target: NBR1 (Neighbor of BRCA1 Gene 1 (NBR1))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NBR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NBR1 Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Brassicaceae family

Immunogen: UNA2 domain of NBR1 of Arabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690

Application Details

Application Notes: 1 : 500 - 1: 5000 with standard ECL (WB)

Comment:

specific extraction method and tissue type needs to be used as described in Minina et al. (2013). Dilution in western blot depends upon amount of NBR1 in the sample.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for NBR1

Target: NBR1 (Neighbor of BRCA1 Gene 1 (NBR1))

Alternative Name: Autophagy substrate NBR1

Background: NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin. Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.

Molecular Weight: 75 | 100 kDa

UniProt: Q9SB64

Pathways: Autophagy