NBR1 antibody

Catalog No. ABIN4966110

The Rabbit Polyclonal anti-NBR1 antibody (ABIN4966110) specifically detects NBR1 in WB and IL. The antibody is reactive with Arabidopsis thaliana and Physcomytrium Patens samples.

Quick Overview for NBR1 antibody (ABIN4966110)

Target: NBR1 (Neighbor of BRCA1 Gene 1 (NBR1))

Reactivity: Arabidopsis thaliana, Physcomytrium Patens

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NBR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details anti-NBR1 Antibody

Purpose: Anti-NBR1 | Autophagy substrate NBR1

Cross-Reactivity (Details): Arabidopsis thaliana, Physcomitrium patens
Not reactive in: Citrus sinensis, Nicotiana tabacum

Predicted Reactivity: Brassicaceae family,Zea mays

Purification: Serum

Immunogen: UBA2 domain of NBR1 of Arabidopsis thaliana

Application Details

Application Notes: 1: 1000 (IL), 1 : 500-1 : 5000 (WB)

Comment:

specific extraction method and tissue type needs to be used as described in Minina et al. (2013). Dilution in western blot depends upon amount of NBR1 in the sample.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for NBR1

Target: NBR1 (Neighbor of BRCA1 Gene 1 (NBR1))

Alternative Name: Autophagy substrate NBR1

Background: NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin. Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.

Molecular Weight: 75 | 100 kDa

Gene ID: 828571

UniProt: Q9SB64

Pathways: Autophagy