BRI1 antibody
BRI1
Reactivity: Arabidopsis thaliana
WB, IP
Host: Rabbit
Polyclonal
unconjugated
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- Target See all BRI1 products
- BRI1 (Protein Brassinosteroid Insensitive 1 (BRI1))
- Reactivity
- Arabidopsis thaliana
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This BRI1 antibody is un-conjugated
- Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Cross-Reactivity (Details)
- Not reactive in: Hordeum vulgare
- Predicted Reactivity
- Brassica rapa
- Immunogen
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BRI1 protein, Uniprot: O22476, TAIR: AT4G39400
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- Application Notes
- 1: 5000 (WB)
- Comment
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Antibody was tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans. For IP: 15 μl GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 μL of sterile water.
- Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- BRI1 (Protein Brassinosteroid Insensitive 1 (BRI1))
- Alternative Name
- Brassinosteroid insensitive 1 (BRI1) (BRI1 Products)
- Synonyms
- ATBRI1 antibody, BIN1 antibody, BR INSENSITIVE 1 antibody, BRASSINOSTEROID INSENSITIVE 1 antibody, CABBAGE 2 antibody, CBB2 antibody, DWARF 2 antibody, DWF2 antibody, F23K16.30 antibody, F23K16_30 antibody, Leucine-rich receptor-like protein kinase family protein antibody, BRI1 antibody
- Background
- BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1) is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase
- Molecular Weight
- above 130 kDa (due to N-glycosylation)
- UniProt
- O22476
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