MDH2 antibody

Catalog No. ABIN4966235

The Rabbit Polyclonal anti-MDH2 antibody has been validated for WB and IP. It is suitable to detect MDH2 in samples from Zea mays.

Quick Overview for MDH2 antibody (ABIN4966235)

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Reactivity: Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-MDH2 Antibody

Predicted Reactivity: Arabidopsis thaliana, Brachypodium distachyon, Citrus sinensis, Coffea canephora, Cucumis sativus, Glycine max, Gossypium raimondii , Hordeum vulgare, Jatropha curcas, Leersia perrieri, Morus notabilis, Oryza sativa, Phaseolus vulgaris , Populus trichocarpa, Prunus persica, Ricinus communis, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Zostera marina, Vitis vinifera

Immunogen: Recombinant MDH2 of Zea mays, UniProt: B4FZU8

Application Details

Application Notes: 1:1000 (WB)

Comment:

Immunoprecipitation was performed by using Dynabeads ® Protein A: briefly 100 μl suspension was washed with 200 μl TTBS (Tris Buffered saline, 50 mM Tris-HCl pH 7.6 and 165 mM NaCl with 0.1% Tween 80) using the magnetic stands for concentrating the magnetic beads. After wash the beads were preincubated with 20 μl primary antibodies in 180 μl TTBS at room temperature for 30 minutes (minimum 15 minues). A first wash was followed afterwards with 200 μl TTBS and hence a real incubation with 200 μl plant extract (supernatant 20,000 x g for 3 min.), 200μl of TTBS and further 50 μl YeastBuster reagent (Novagen) containing a mixture of detergents to break and solubilize the mitochondria membrane. This incubation at room temperature was allowed to be under mild shaking to allow the beads to be in suspension. Hence supernatant was aspirated away by the use of the magnetic stand and two further washesing steps with 200 μl TTBS were performed prior mixing with 100 μl SDS-Sample buffer.

Restrictions: For Research Use only

Handling

Reconstitution: For reconstitution add 50 μL of sterile water.

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for MDH2

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Alternative Name: Malate dehydrogenase 2 (mitochondrial) (MDH2)

Background: Malate dehydrogenase (EC=1.1.1.37) is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate. This reaction is part of many metabolic pathways, including the citric acid cycle. Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. The protein is highly expressed in: young panicles and immature seeds, while its levels in roots and leaves are low. Alternative name: PP37.

Molecular Weight: 35 | 35 kDa

UniProt: B4FZU8