HY5 antibody

Catalog No. ABIN4966286

The Rabbit Polyclonal anti-HY5 antibody is suitable to detect HY5 in samples from Arabidopsis thaliana. It has been validated for WB.

Quick Overview for HY5 antibody (ABIN4966286)

Target: HY5 (Transcription Factor HY5 (HY5))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-HY5 Antibody

Purpose: Anti-HY5 | Protein long hypocotyl 5

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Citrus reticulata, Hordeum vulgare, Marchantia polymorhpa, Oryza sativa, Pisum sativum, Populus sp., Solanum lycopersicum, Triticum aestivum, Zea mays

Predicted Reactivity: Brassica pekinensis

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide

Application Details

Application Notes: 1: 500 to 1 : 1000 (WB)

Comment:

This antibody detects well recombinant HY5 in Nicotiana benthamiana (image below) while a signal in endogenous total cell extract of Arabidopsis thaliana has not been optimised in applied conditions (as below).Using nuclear fraction for detection of endogenous protein is recommened, however it has not been optimized yet.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for HY5

Target: HY5 (Transcription Factor HY5 (HY5))

Alternative Name: Protein long hypocotyl 5 (HY5)

Background: HY5 (Protein long hypocotyl 5) is a light-regulated transcription factor that promotes photomorphogenesis in light. Involved in the blue light specific pathway. Heterodimer. Expressed in root, hypocotyl, cotyledon, leaf, stem and floral organs. Localized in nucleus. Alternative names: Protein LONG HYPOCOTYL 5, bZIP transcription factor 56, AtbZIP56.

Molecular Weight: 18.5 kDa

Gene ID: 830996

UniProt: O24646