Pyruvate Orthophosphate Dikinase (PPDK) (AA 72-947) antibody

Catalog No. ABIN4966289

The Rabbit Polyclonal anti-Pyruvate Orthophosphate Dikinase (PPDK) antibody is suitable to detect Pyruvate Orthophosphate Dikinase (PPDK) in samples from Arabidopsis thaliana, Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus and Zea mays. It has been validated for WB.

Quick Overview for Pyruvate Orthophosphate Dikinase (PPDK) (AA 72-947) antibody (ABIN4966289)

Target: Pyruvate Orthophosphate Dikinase (PPDK)

Reactivity: Arabidopsis thaliana, Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Zea mays

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details

Binding Specificity: AA 72-947

Purpose: Anti-PPDK | Pyruvate orthophosphate dikinase

Cross-Reactivity (Details): Arabidopsis thaliana,Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Zea mays
Not reactive in: Cucumis sativus

Predicted Reactivity: Hordeum vulagre,Kalanchoe fedtschenkoi

Purification: Immunogen affinity purified serum

Immunogen: Purified recomibinant enzyme consisting of residues 72-947 of Zea mays

Application Details

Application Notes: 1 : 25 000 (WB)

Comment:

PPDK levels inr C3 plants like Arabidopsis thaliana and Hordeum vulgare are very low and PPDK protein is very dilute in most tissues of C3 plants. To perform detection in C3 plants leaf proteins needs to be concentrated before western blot, Chastain et al. (2002).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water in 40 % glycerol to a final protein concentration of 100 ng/μl

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: Pyruvate Orthophosphate Dikinase (PPDK)

Background: PPDK (Pyruvate, phosphate dikinase 1) is involved in formation of phosphoenolpyruvate and activated by light-induced dephosphorylation. Inhibited by dark-induced phosphorylation. PPDK is a low-abundance enzyme in C3 plants while it is a key enzyme of C4 photosynthesis.

Molecular Weight: 102 | 95 kDa

Gene ID: 827226

UniProt: O23404