RNA Polymerase beta Subunit (Chloroplast) (RpoB) antibody

Catalog No. ABIN4966308

The Rabbit Polyclonal anti-RNA Polymerase beta Subunit (Chloroplast) (RpoB) antibody (ABIN4966308) specifically detects RNA Polymerase beta Subunit (Chloroplast) (RpoB) in WB. The antibody is reactive with Arabidopsis thaliana samples.

Quick Overview for RNA Polymerase beta Subunit (Chloroplast) (RpoB) antibody (ABIN4966308)

Target: RNA Polymerase beta Subunit (Chloroplast) (RpoB)

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Alloteropsis semialata, Coleataenia prionitis, Digitaria exilis, Echinochloa crus-galli var. crus-galli , Eragrostis tef, Microlaena stipoides, Miscanthus sacchariflorus, Oryza sativa, Phragmites australis, Potamophila parviflora, Rhynchoryza subulata, Saccharum officinarum, Setaria italica, Sorghum bicolor, Stipa lipskyi, Sporobolus michauxianus, Triticum aestivum

Immunogen: His-tagged, highly conserved fragment of Zea mays RpoB gi|540067377|gb|AGV02730.1| RNA polymerase beta subunit (chloroplast) [Zea mays subsp. mays], UniProt: A0A059Q6W3

Application Details

Application Notes: 1: 500 (WB)

Comment:

Antibody does not work on total cell extracts. Stromal fraction has to be used.This antibody is detecting recombinant RpoB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details

Target: RNA Polymerase beta Subunit (Chloroplast) (RpoB)

Background: RpoB (RNA polymerase beta subunit (chloroplast)) is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonuceloside triphosphates as substrates.

Molecular Weight: 121 kDa

UniProt: P50546