Transketolase (Chloroplastic) (TKL1) antibody

Catalog No. ABIN4966343

The Rabbit Polyclonal anti-Transketolase (Chloroplastic) (TKL1) antibody is suitable to detect Transketolase (Chloroplastic) (TKL1) in samples from Arabidopsis thaliana and Pea. It has been validated for IP and WB.

Quick Overview for Transketolase (Chloroplastic) (TKL1) antibody (ABIN4966343)

Target: Transketolase (Chloroplastic) (TKL1)

Reactivity: Arabidopsis thaliana, Pea

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Purpose: Anti-TKL1 | transketolase (chloroplastic)

Cross-Reactivity (Details): Arabidopsis thaliana, Pisum sativum

Predicted Reactivity: Cucumis sativus,Synechococcus elongatus sp PCC 7942

Purification: Serum

Immunogen: Purified

Application Details

Application Notes: 1 : 2000 (WB)

Comment:

TKL1 has MW of 79.28 kDa with and 73.45 kDa without presequence, about 72 kDa on SDS gelAntibody works on whole leaf extracts and isolated chloroplasts and is also recognizing recombinant TKL protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 45 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: Transketolase (Chloroplastic) (TKL1)

Background: TKL-1 (Transketolase-1, chloroplastic) is involved in the pathway of Calvin cycle. This enzyme is catalyzing the reversible transfer of a two-carbon ketol group from fructose-6-phopshate or sedoheptuloze-7-phosphate to glyceraldehyde-3-phosphate to yield xylulose-5-phosphate and erythrose-4-phosphate or ribose-5-phosphate. AtTKL1 is expressed in highest levels in photosynthetic tissue, while AtTKL2 is expressed mainly during embryo development. Alternative name: TK.

Molecular Weight: 72 kDa

Gene ID: 825246

UniProt: Q8RWV0