Fertilization-Independent Endosperm antibody
FIE
Reactivity: Arabidopsis thaliana
WB
Host: Rabbit
Polyclonal
unconjugated
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- Target See all Fertilization-Independent Endosperm (FIE) products
- Fertilization-Independent Endosperm (FIE) (Polycomb Group Protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE))
- Reactivity
- Arabidopsis thaliana
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity are currently known
- Predicted Reactivity
- Arabidopsis thaliana, Triticum aestivum
- Immunogen
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana FIE protein sequence, UniProt: Q9LT47, TAIR: At3g20740
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- Application Notes
- 1 : 1000 with ECL (WB)
- Comment
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This antibody so far has been confirmed to detect recombinant FIE protein, GFP-tagged. Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous FIE.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 μL of sterile water.
- Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- Fertilization-Independent Endosperm (FIE) (Polycomb Group Protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE))
- Alternative Name
- fertilization-independent endosperm (FIE) (FIE Products)
- Synonyms
- FERTILIZATION-INDEPENDENT ENDOSPERM antibody, FERTILIZATION-INDEPENDENT ENDOSPERM 1 antibody, FIE1 antibody, FIS3 antibody, Transducin/WD40 repeat-like superfamily protein antibody, FIE antibody
- Background
- FIE (fertilization-independent endosperm) belongs to polycomb group (PcG) protein which act by forming multiprotein complexes, required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.
- Molecular Weight
- 41 kDa
- UniProt
- Q9LT47
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