ATG12 antibody (N-Term)

Catalog No. ABIN497963

The Rabbit Polyclonal anti-ATG12 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect ATG12 in samples from Human, Mouse and Rat.

Quick Overview for ATG12 antibody (N-Term) (ABIN497963)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ATG12 Antibody

Binding Specificity: N-Term

Specificity: Recognizes APG12/ATG12.

Purification: Affinity Chromatography purified via peptide column.

Immunogen: A 16 amino acid peptide from near the amino terminus of Human ATG12.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: ATG12 antibody can be used for the detection of ATG12 at 0.5-1 μg/mL. Immunohistochemistry on Paraffin Sections. Positive Control. Human Brain Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12 / APG12

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.Synonyms: APG12-like, APG12L, Autophagy-related protein 12, Ubiquitin-like protein ATG12

Gene ID: 9140

NCBI Accession: NP_004698

UniProt: O94817

Pathways: Autophagy