ATG12 antibody (Middle Region)

Catalog No. ABIN497964

This anti-ATG12 antibody is a Rabbit Polyclonal antibody detecting ATG12 in WB, IHC (p) and EIA. Suitable for Human, Mouse and Rat.

Quick Overview for ATG12 antibody (Middle Region) (ABIN497964)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ATG12 Antibody

Binding Specificity: Middle Region

Specificity: Recognizes APG12/ATG12.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat

Purification: Affinity Chromatography purified via peptide column

Immunogen: A 15 amino acid peptide from near the center of Human ATG12

Isotype: IgG

Application Details

Application Notes: Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12 / APG12

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.Synonyms: APG12-like, APG12L, Autophagy-related protein 12, Ubiquitin-like protein ATG12

Gene ID: 9140

NCBI Accession: NP_004698

UniProt: O94817

Pathways: Autophagy