Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 466-496 amino acids from the C-terminal region of Human Lamin B1. Genename: LMNB1
ELISA: 1/1,000Western blotting: 1/50approx. 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Lamin B1 is one of the two B type proteins, B1.Synonyms: LMN2, LMNB, LMNB-1, LMNB1, Lamin-B1, Nuclear Envelope Marker