ATG10 antibody (C-Term)

Catalog No. ABIN497986

This Rabbit Polyclonal antibody specifically detects ATG10 in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATG10 antibody (C-Term) (ABIN497986)

Target: ATG10 (Autophagy Related 10 (ATG10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG10 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ATG10 Antibody

Binding Specificity: C-Term

Specificity: Recognizes APG10L/ATG10 (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat

Purification: Immunoaffinity Chromatography

Immunogen: ATG10 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of Human ATG10 (Genbank accession No. EAW95884).

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: ATG10 antibody can be used for the detection of ATG10 at 0.5-1 μg/mL. Positive Control: SK-N-SH Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ATG10

Target: ATG10 (Autophagy Related 10 (ATG10))

Alternative Name: APG10L / ATG10

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is ATG10, an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.Synonyms: Autophagy-related protein 10

Gene ID: 83734

NCBI Accession: NP_001124500

UniProt: Q9H0Y0

Pathways: ER-Nucleus Signaling, Autophagy