RAP1 antibody (C-Term)

Catalog No. ABIN498023

The Goat Polyclonal anti-RAP1 antibody is suitable to detect RAP1 in samples from Human, Mouse, Rat, Dog and Cow. It has been validated for WB and EIA.

Quick Overview for RAP1 antibody (C-Term) (ABIN498023)

Target: RAP1 (TERF2IP) (Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP))

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This RAP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RAP1 Antibody

Binding Specificity: C-Term

Sequence: C-GAQNVARRIE FRKK

Specificity: Recognizes RAP1/TERF2IP (C-term).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Bovine, Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_061848.2. Genename: TERF2IP

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/64000. Western blot: 0.1-0.3 μg/mL. Approx 60 kDa band observed in Human Bone Marrow, LymphNode and Tonsil lysates (calculated MW of 44.0 kDa according to NP_061848.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RAP1

Target: RAP1 (TERF2IP) (Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP))

Alternative Name: TERF2IP / RAP1

Background: RAP1 is located at telomeres and affects telomere length. However, unlike yeast RAP1 that binds telomeric DNA directly, human RAP1 is recruited to telomeres by TRF2.Synonyms: PP8000, TRF2-interacting telomeric protein Rap1, Telomeric repeat-binding factor 2-interacting protein 1

Gene ID: 54386

NCBI Accession: NP_061848

UniProt: Q9NYB0

Pathways: Cell Division Cycle, Telomere Maintenance