HOXA4 antibody (Internal Region)

Catalog No. ABIN498071

The Goat Polyclonal anti-HOXA4 antibody is suitable to detect HOXA4 in samples from Human and Dog. It has been validated for WB and EIA.

Quick Overview for HOXA4 antibody (Internal Region) (ABIN498071)

Target: HOXA4 (Homeobox A4 (HOXA4))

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This HOXA4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-HOXA4 Antibody

Binding Specificity: Internal Region

Sequence: C-QAKGPAHGLH ASH

Specificity: Recognizes HOXA4.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002132.3. Genename: HOXA4

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/32000. Western blot: 0.1-0.3 μg/mL. Detects a band of Approx 36 kDa in Human Colon lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HOXA4

Target: HOXA4 (Homeobox A4 (HOXA4))

Alternative Name: HOXA4 / HOX1D

Background: The Hox homeobox genes encode proteins that are transcriptional regulators with an established role in embryonic development. HoxA4 (homeobox A4), also known as HOX1D or HOX1, is a 320 amino acid protein that localizes to the nucleus and contains one homeobox DNA-binding domain. Expressed in the embryonic nervous system, HoxA4 functions as a sequence-specific DNA-binding transcription factor that is part of a regulatory mechanism that provides cells with positional identities during development. Via its ability to bind DNA, HoxA4 plays an important role in the regulation of gene expression, as well as morphogenesis and differentiation. The gene encoding HoxA4 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5 % of the human genome. Defects in some of the genes localized to chromosome 7 have been linked to osteogenesis imperfecta, Williams-Beuren syndrome, Pendred syndrome, lissencephaly, citrullinemia and Shwachman-Diamond syndrome.Synonyms: Homeobox protein Hox-A4, Hox-1.4, Hox-1D

Gene ID: 3201

NCBI Accession: NP_002132

UniProt: Q00056