SOX3 antibody (C-Term)

Catalog No. ABIN498075

The Goat Polyclonal anti-SOX3 antibody (ABIN498075) specifically detects SOX3 in WB, IHC (p) and EIA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for SOX3 antibody (C-Term) (ABIN498075)

Target: SOX3 (SRY (Sex Determining Region Y)-Box 3 (SOX3))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SOX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SOX3 Antibody

Binding Specificity: C-Term

Sequence: C-DAASPLPGGR LHGVH

Specificity: This antibody recognizes SOX3 (C-term).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide corresponding to the C-Terminus of Human SOX3 (NP_005625.2). Genename: SOX3

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.5-1.5 μg/mL. Detects a band of Approx 42 kDa in Human Brain (Cerebellum, Cerebral Cortex and FrontalCortex) lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat, Canine (Dog). Add. Information: Calculated Molecular Weight: 45.2 kDa according to NP_005625.2

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SOX3

Target: SOX3 (SRY (Sex Determining Region Y)-Box 3 (SOX3))

Alternative Name: SOX3

Background: SOX3 is also known as SRY related HMG BOX gene 3. All SOX proteins have a single HMG box. Humans with mutations in SOX3 have panhypopituitarism. Conditional disruption of SOX3 in mice demonstrates that anterior pituitary development depends on SOX3 expression in the overlying neural ectoderm.Synonyms: GHDX, MRGH, SOXB, SRY (sex determining region Y)-box 3, SRY-box 3, Transcription factor SOX-32

Molecular Weight: 45.2kDa according to NP_005625.2

Gene ID: 6658

NCBI Accession: NP_005625

UniProt: P41225