ICAM-3/CD50 antibody

Catalog No. ABIN498110

The Rabbit Polyclonal anti-ICAM-3/CD50 antibody is suitable to detect ICAM-3/CD50 in samples from Human. It has been validated for WB and IF.

Quick Overview for ICAM-3/CD50 antibody (ABIN498110)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICAM-3/CD50 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ICAM-3/CD50 Antibody

Specificity: This antibody detects endogenous levels of CD50 protein.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name: CD50 / ICAM3

Background: All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA1 protein. ICAM3 is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Upon stimulation by a physiological stimulus it becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated, glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.Synonyms: CDw50, ICAM-3, ICAM-R, Intercellular adhesion molecule 3

Molecular Weight: approx. 59 kDa

Gene ID: 3385

NCBI Accession: NP_002153

UniProt: P32942