KSR1 antibody

Catalog No. ABIN498178

This Rabbit Polyclonal antibody specifically detects KSR1 in WB, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for KSR1 antibody (ABIN498178)

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KSR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KSR1 Antibody

Specificity: This antibody detects endogenous levels of KSR protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for KSR1

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Alternative Name: Kinase Suppressor of Ras 1

Background: Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 has intrinsic kinase activity towards serine/threonine residues and is widely expressed in many tissue types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood. Two proteins putatively involved in this process are Ksr-1 and Tak1. Ksr-1 (kinase suppressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known. Tak1 (TGFβ-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFβ stimulation.Synonyms: KSR, KSR-1, KSR1

Molecular Weight: approx. 115 kDa

Gene ID: 8844

NCBI Accession: NP_055053

UniProt: Q8IVT5