Separase antibody

Catalog No. ABIN498181

This Rabbit Polyclonal antibody specifically detects Separase in WB, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Separase antibody (ABIN498181)

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Separase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Separase Antibody

Specificity: This antibody detects endogenous levels of Separase protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for Separase

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Alternative Name: Separin / ESPL1

Background: Separase is a cysteine protease that triggers anaphase in all eukaryotes by participating in separation of sister chromatids during mitosis. Once activated, separase hydrolyzes the SCC1 subunit of cohesin, the chromosomal protein complex responsible for sister chromatid cohesion. Separase and cohesin are highly conserved from yeasts to humans. When the cell is not dividing, separase is prevented from cleaving cohesin through its association with another protein, securin. When anaphase is signaled, the securin is ubiquitinated and hydrolyzed by APC/cyclosome, releasing the active separase. Separase is transiently activated between the two meioses and may also be involved in homolog separation.Synonyms: Caspase-like protein ESPL1, ESP1, Extra spindle poles-like 1 protein, KIAA0165, Separase

Molecular Weight: approx. 230 kDa

Gene ID: 9700

NCBI Accession: NP_036423

UniProt: Q14674