Connexin 43/GJA1 antibody

Catalog No. ABIN498197

The Rabbit Polyclonal anti-Connexin 43/GJA1 antibody has been validated for IF and IHC (p). It is suitable to detect Connexin 43/GJA1 in samples from Human.

Quick Overview for Connexin 43/GJA1 antibody (ABIN498197)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Connexin 43/GJA1 Antibody

Specificity: This antibody detects endogenous levels of Connexin 43 protein.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity-Chromatography using epitope-specific immunogen

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: GJA1 / Cx43

Background: The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. In Src transformed cells, there is a decrease in gap junctional communication, which appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on Tyr 265, resulting in a stable interaction between both proteins, which leads to inhibition of gap junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the ab-sence of Src kinases and on both serine and tyrosine in cells expressing Src kinases, such as c-Src and/or pp60v-Src. In human vascular endothelial cells, connexin 43 is posttranslationally modified during mitosis. Mitosis-specific phosphorylation of connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of connexin 43.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein

Molecular Weight: approx. 43 kDa

Gene ID: 2697

NCBI Accession: NP_000156

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization