PKC epsilon antibody

Catalog No. ABIN498251

The Rabbit Polyclonal anti-PKC epsilon antibody is suitable to detect PKC epsilon in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for PKC epsilon antibody (ABIN498251)

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC epsilon antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PKC epsilon Antibody

Specificity: This antibody detects endogenous levels of PKC ε protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for PKC epsilon

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Alternative Name: PRKCE

Background: Members of the protein kinase C family fall into three categories: conventional (PKC alph,a beta I, beta II, gamma), novel (delt,a epsilon, et,a mu, theta), and atypical (lamd,a zeta). Conventional PKC isoforms are dependent on calcium and diacylglycerol (DAG) for their activity, whereas novel PKC isoforms are calcium dependent, and atypical PKC isoforms are calcium and DAG independent.Synonyms: PKC epsilon, PKC-epsilon, PKCE, Protein kinase C epsilon type

Molecular Weight: approx. 83 kDa

Gene ID: 5581

NCBI Accession: NP_005391

UniProt: Q02156

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling