ADRA2A antibody

Catalog No. ABIN498305

The Rabbit Polyclonal anti-ADRA2A antibody is suitable to detect ADRA2A in samples from Human, Rat and Mouse. It has been validated for WB, IF and IHC (p).

Quick Overview for ADRA2A antibody (ABIN498305)

Target: ADRA2A (Alpha-2A Adrenergic Receptor (ADRA2A))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRA2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ADRA2A Antibody

Specificity: This antibody detects endogenous levels of AR alpha-2A protein. (region surrounding Arg361)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ADRA2A

Target: ADRA2A (Alpha-2A Adrenergic Receptor (ADRA2A))

Alternative Name: alpha-2A Adrenergic Receptor

Background: This study investigates the involvement of alpha2-adrenergic receptors (AR) in mouse brain induced by a low dose of methamphetamine (METH, 2 mg/kg). Immunohistochemical studies show that alpha2A-AR increased in the dentate gyrus area of the hippocampus 24 h after five repeated administrations of METH. The hippocampal alpha2A-AR proteins rose 3.2-fold when compared to the saline-administered mice. The other adrenergic receptor, apha1D-AR, were not changed by the treatment. Moreover, alphao-subunits of GTP-binding proteins (Galphao), one of the downstream molecules of alpha2A-AR, was also increased by the treatment. These suggest that the repeated administration of low-doses of METH causes quantitative changes of the signaling of alpha2A-AR in the mouse hippocampus.Synonyms: ADRA2A, ADRA2R, ADRAR, Alpha-2A adrenoceptor, Alpha-2A adrenoreceptor

Molecular Weight: approx. 48 kDa

Gene ID: 150

NCBI Accession: NP_000672

UniProt: P08913

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Negative Regulation of Transporter Activity