MELK antibody

Catalog No. ABIN498341

The Rabbit Polyclonal anti-MELK antibody is suitable to detect MELK in samples from Human. It has been validated for WB, IF and IHC (p).

Quick Overview for MELK antibody (ABIN498341)

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MELK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MELK Antibody

Specificity: This antibody detects endogenous levels of MELK protein. (region surrounding asn449)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 with 0.05 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MELK

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Alternative Name: MELK

Background: Maternal Embryonic Leucine Zipper Kinase (KIAA0175, HPK38) or MELK, a new member of the Snf1/AMPK family of kinases, encodes a protein with a kinase catalytic domain and a leucine zipper motif consisting of a periodic repetition of leucine residues at every seventh residue located within the N-terminal catalytic domain. This motif has been observed in myriad DNAbinding proteins and is presumed to be involved in protein-DNA interactions, and potentially protein-protein interactions. Research predicts that the gene product of MELK plays a role in the signal transduction events in the egg and early embryo. Mouse and human MELK proteins share 95 % sequence identity in the kinase domain and northern blot analysis in mouse indicates that MELK expression is restricted to spermatogonia in the testis and to oocytes in the ovary.Synonyms: KIAA0175, Maternal embryonic leucine zipper kinase, hMELK

Molecular Weight: approx. 75 kDa

Gene ID: 9833

NCBI Accession: NP_055606

UniProt: Q14680