14-3-3 zeta antibody

Catalog No. ABIN498366

The Rabbit Polyclonal anti-14-3-3 zeta antibody (ABIN498366) specifically detects 14-3-3 zeta in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for 14-3-3 zeta antibody (ABIN498366)

Target: 14-3-3 zeta (YWHAZ)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 zeta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-14-3-3 zeta Antibody

Specificity: This antibody detects endogenous levels of 14-3-3 ζ protein. (region surrounding Ala79)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: 14-3-3 Protein zeta/delta

Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, theta, epsilon, zeta, sigma and nu. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzyme activity etc.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ

Molecular Weight: approx. 28 kDa

Gene ID: 7534

NCBI Accession: NP_001129171

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location