CYB5R3 antibody

Catalog No. ABIN498381

The Rabbit Polyclonal anti-CYB5R3 antibody (ABIN498381) specifically detects CYB5R3 in WB, IF and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for CYB5R3 antibody (ABIN498381)

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYB5R3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CYB5R3 Antibody

Specificity: This antibody detects endogenous levels of Cytochrome b5protein. (region surrounding Gly82)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CYB5R3

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Alternative Name: CYB5R3

Background: Cytochrome b5 is a membrane-bound member of the cytochrome b family. A heme protein that functions as an electron carrier for many membrane-bound oxygenases, cytochrome b5 possesses two heme groups, which are not covalently attached to the protein. Two isoforms of cytochrome b5, a microsomal membrane-bound form and a cytoplasmic form, are produced by alternative splicing. Mutations in cytochrome b5 are associated with Leber's hereditary optic neuropathy and with myopathy.Synonyms: B5R, DIA1, Diaphorase-1, NADH-cytochrome b5 reductase 3

Molecular Weight: approx. 15 kDa

Gene ID: 1727

NCBI Accession: NP_000389

UniProt: P00387

Pathways: SARS-CoV-2 Protein Interactome