CDC7 antibody

Catalog No. ABIN498406

This Rabbit Polyclonal antibody specifically detects CDC7 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CDC7 antibody (ABIN498406)

Target: CDC7 (Cell Division Cycle 7 (CDC7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDC7 Antibody

Specificity: This antibody detects endogenous levels of Cdc7 protein. (region surrounding Phe35)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDC7

Target: CDC7 (Cell Division Cycle 7 (CDC7))

Alternative Name: CDC7

Background: DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.Synonyms: CDC7-related kinase, CDC7L1, Cell division cycle 7-related protein kinase, HsCdc7, huCdc7

Molecular Weight: approx. 70 kDa

Gene ID: 8317

NCBI Accession: NP_001127891

UniProt: O00311

Pathways: Mitotic G1-G1/S Phases, DNA Replication