AKAP1 antibody

Catalog No. ABIN498433

This Rabbit Polyclonal antibody specifically detects AKAP1 in WB, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for AKAP1 antibody (ABIN498433)

Target: AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AKAP1 Antibody

Specificity: This antibody detects endogenous levels of AKAP149 protein. (region surrounding asn306)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity-Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for AKAP1

Target: AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))

Alternative Name: AKAP1

Background: The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. This family of proteins has been designated A-kinase anchoring proteins (AKAP). Members of this family, including MAP2 (microtubule-associated protein 2), neuronally expressed AKAP 79 and AKAP 150, and the DNA binding AKAP 95, display differential tissue specificity and localization. Evidence suggests that AKAP 79 and AKAP 150 are both capable of anchoring PKA to postsynaptic densities (PSD), which are a network of proteins located on the internal surfaces of excitatory synapses.Synonyms: A kinase anchor protein 1 mitochondrial, A-kinase anchor protein 149 kDa, AKAP-1, AKAP149, Dual specificity A-kinase-anchoring protein 1, PRKA1, Protein kinase A-anchoring protein 1, Spermatid A-kinase anchor protein 84

Molecular Weight: approx. 90 kDa

Gene ID: 8165

NCBI Accession: NP_003479

UniProt: Q92667

Pathways: Synaptic Membrane