KRT14 antibody

Catalog No. ABIN498520

This Rabbit Polyclonal antibody specifically detects KRT14 in IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for KRT14 antibody (ABIN498520)

Target: KRT14 (Keratin 14 (KRT14))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT14 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KRT14 Antibody

Specificity: This detects endogenous levels of Cytokeratin 14 protein.(region surrounding Tyr151)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Cytokeratin 14

Background: Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed in pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85 % of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10-nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and in addition they aid in the characterization of malignant tumors. In Bowen's disease, the characteristic malignancy of the epidermis exhibits distinct expression patterns of cytokeratin 14. The gene encoding human cytokeratin 14 maps to chromosome 17q12- 21. Mutations in this gene lead to epidermolysis bullosa simplex, an inheritied skin disorder characterized by skin blistering due to basal keratinocyte fragility.Synonyms: CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14

Molecular Weight: approx. 52 kDa

Gene ID: 3861

NCBI Accession: NP_000517

UniProt: P02533