NEFH antibody

Catalog No. ABIN498522

The Rabbit Polyclonal anti-NEFH antibody (ABIN498522) specifically detects NEFH in IHC (p). The antibody is reactive with Human samples.

Quick Overview for NEFH antibody (ABIN498522)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NEFH Antibody

Specificity: This antibody detects endogenous levels of NF-H protein. (region surrounding Glu972)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: Neurofilament H (200 kD)

Background: Neurofilament-H (for neurofilament heavy polypeptide, or NF-H), a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures, they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase- 3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease, and Alzheimer's disease.Synonyms: (Neuronal Marker), 200 kDa neurofilament protein, KIAA0845, NEFH, NF-H, NFH, Neurofilament triplet H protein, heavy polypeptide

Molecular Weight: approx. 112 kDa

Gene ID: 4744

NCBI Accession: NP_066554

UniProt: P12036