MIP
Reactivity: Human
IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 350
Application Notes
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
AQP0 antibody, CTRCT15 antibody, LIM1 antibody, MIP26 antibody, MP26 antibody, Aqp0 antibody, Cat antibody, Hfi antibody, Lop antibody, Svl antibody, shrivelled antibody, MP22 antibody, aqp0 antibody, lim1 antibody, mp26 antibody, mip26 antibody, MIP antibody, mip1 antibody, zgc:100858 antibody, mip2 antibody, zgc:110015 antibody, major intrinsic protein of lens fiber antibody, major intrinsic protein of lens fiber L homeolog antibody, major intrinsic protein of lens fiber a antibody, major intrinsic protein of lens fiber b antibody, MIP antibody, Mip antibody, mip.L antibody, mip antibody, mipa antibody, mipb antibody
Background
Aquaporins (AQPs) are a large family of integral membrane channel proteins that facilitate the transport of water through the cell membrane. Aquaporins are widely distributed and are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells where it functions not only as a water pore, but it is also involved in fiber-fiber adhesion and is crucial for fiber cell structure and organization. AQP0 contains an additional pore constriction, not seen in any other aquaporin structures, which may be responsible for pore gating. The closed AQP0 pore holds just three water molecules, which are spaced too far apart to form hydrogen bonds with each other. The C-terminal domain of AQP0 undergoes extensive post-translational modification, including many truncations, during lens aging due to the actions of m-Calpain, proteases or non-enzymatic mechanisms. These truncation sites may be involved in the development of cataracts.Synonyms: AQP0, Lens fiber major intrinsic protein, MIP, MIP26, MP26