IL32 antibody

Catalog No. ABIN498668

Product Details anti-IL32 Antibody

Target: IL32 (Interleukin 32 (IL32))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL32 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of IL-32 protein. (region surrounding Lys226)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL32

Target: IL32 (Interleukin 32 (IL32))

Alternative Name: Interleukin-32 / IL32 (IL32 Products)

Synonyms: IL-32alpha antibody, IL-32beta antibody, IL-32delta antibody, IL-32gamma antibody, NK4 antibody, TAIF antibody, TAIFa antibody, TAIFb antibody, TAIFc antibody, TAIFd antibody, interleukin 32 antibody, IL32 antibody

Background: The interleukins make up a large family of well characterized cytokines, primarily of hematopoietic cell origin. The interleukins are secreted by immune cells (mainly macrophages, B cells and T cells) that regulate a wide range of immune system functions. Interleukin-32 (IL-32), also designated Natural Killer (NK) cell transcript 4 (nk4), may play a role in inflammatory responses such as lymphocyte activation. IL-32 is secreted following stimulation with inflammatory cytokines such as IL-1beta and IFN-gamma, and by NK cells after exposure to IL-12 and IL-18. It also induces production of IL-1beta, TNFalpha, MIP-2 and IL-6. Expression of IL-32 increases after activation of T cells by mitogens or activation of NK cells by IL-2. The IL-32 protein contains three potential Nmyristoylation sites, a tyrosine sulfation site, an RGD cellattachment sequence and multiple putative phosphorylation sites. Human IL-32 exists as four isoforms.Synonyms: IL-32, NK4, Natural killer cells protein 4, TAIF, Tumor necrosis factor alpha-inducing factor

Molecular Weight: approx. 27 kDa

Gene ID: 9235

NCBI Accession: NP_001012649

UniProt: P24001