IL32 antibody (Interleukin 32)

Details for Product anti-IL32 Antibody No. ABIN498668
Antigen
Reactivity
Human
135
23
4
Host
Rabbit
115
19
1
Clonality
Polyclonal
Conjugate
This IL32 antibody is un-conjugated
7
6
5
4
4
3
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
103
62
44
18
12
7
6
5
4
3
2
2
1
1
1
1
Options
Specificity This antibody detects endogenous levels of IL-32 protein. (region surrounding Lys226)
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE
Antigen
Alternative Name Interleukin-32 / IL32 (IL32 Antibody Abstract)
Background The interleukins make up a large family of well characterized cytokines, primarily of hematopoietic cell origin. The interleukins are secreted by immune cells (mainly macrophages, B cells and T cells) that regulate a wide range of immune system functions. Interleukin-32 (IL-32), also designated Natural Killer (NK) cell transcript 4 (nk4), may play a role in inflammatory responses such as lymphocyte activation. IL-32 is secreted following stimulation with inflammatory cytokines such as IL-1beta and IFN-gamma, and by NK cells after exposure to IL-12 and IL-18. It also induces production of IL-1beta, TNFalpha, MIP-2 and IL-6. Expression of IL-32 increases after activation of T cells by mitogens or activation of NK cells by IL-2. The IL-32 protein contains three potential Nmyristoylation sites, a tyrosine sulfation site, an RGD cellattachment sequence and multiple putative phosphorylation sites. Human IL-32 exists as four isoforms.Synonyms: IL-32, NK4, Natural killer cells protein 4, TAIF, Tumor necrosis factor alpha-inducing factor
Molecular Weight approx. 27 kDa
Gene ID 9235
NCBI Accession NP_001012649
UniProt P24001
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Image no. 1 for anti-Interleukin 32 (IL32) antibody (ABIN498668) Western blot (WB) analyzes of IL-32 antibody in extracts from HepG2 cells.
Image no. 2 for anti-Interleukin 32 (IL32) antibody (ABIN498668) Immunohistochemistry (IHC) analyzes of IL-32 antibody in paraffin-embedded human lung...