SNAIL antibody (pSer246)

Catalog No. ABIN498696

The Rabbit Polyclonal anti-SNAIL antibody (ABIN498696) specifically detects SNAIL in WB, IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for SNAIL antibody (pSer246) (ABIN498696)

Target: SNAIL (SNAI1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAIL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SNAIL Antibody

Binding Specificity: pSer246

Specificity: This antibody detects endogenous levels of p-SNAI 1 protein, only when phosphorylated at Ser246.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SNAIL

Target: SNAIL (SNAI1)

Alternative Name: SNAI1

Background: The SNAIL family of developmental regulatory proteins is a group of widely conserved zinc-finger proteins that regulate transcription and include the mammalian proteins SLUG, SNAI 1, the human homolog of DrosophilaSNAIL,and Smuc. SNAI 1 and SLUG are expressed in placenta and adult heart, liver and skeletal muscle. SNAI 1, and the corresponding mouse homolog Sna,each contain three classic zinc fingers and one atypical zinc finger, while SLUG contains five zinc finger regions and a transcriptional repression domain at the amino terminus, which enables SLUG to act as a negative regulator of gene expression. SLUG is implicated in the generation and migration of neural crest cells in human embryos and also contributes to limb bud development.Synonyms: Protein snail homolog 1, SNAH, Zinc finger protein SNAI1

Molecular Weight: approx. 29 kDa

Gene ID: 6615

NCBI Accession: NP_005976

UniProt: O95863

Pathways: Negative Regulation of intrinsic apoptotic Signaling