Cofilin antibody (pSer3)

Catalog No. ABIN498706

This Rabbit Polyclonal antibody specifically detects Cofilin in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cofilin antibody (pSer3) (ABIN498706)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cofilin Antibody

Binding Specificity: pSer3

Specificity: This antibody detects endogenous levels of Cofilin protein only when phosphorylated at Ser3.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: Cofilin-1

Background: Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly, essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonized zymosan. LIM kinase 1 (LIMK-1), a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerize F-Actin.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18

Molecular Weight: approx. 19 kDa

Gene ID: 1072

NCBI Accession: NP_005498

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events