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GSK3 alpha antibody (Glycogen Synthase Kinase 3 alpha) (pSer21) Primary Antibody

GSK3a Reactivity: Human, Mouse, Rat IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN498788
$513.70
Plus shipping costs $45.00
0.1 mg
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Available
  • Target
    GSK3 alpha (GSK3a)
    Binding Specificity
    • 36
    • 20
    • 17
    • 17
    • 11
    • 8
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer21
    Reactivity
    • 106
    • 87
    • 68
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    Human, Mouse, Rat
    Host
    • 119
    • 23
    • 2
    Rabbit
    Clonality
    • 119
    • 27
    Polyclonal
    Conjugate
    • 75
    • 11
    • 11
    • 8
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GSK3 alpha antibody is un-conjugated
    Application
    • 108
    • 72
    • 70
    • 19
    • 14
    • 13
    • 13
    • 12
    • 7
    • 3
    • 3
    • 3
    • 2
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Specificity
    This antibody detects endogenous levels of p-GSK3alpha protein only when phosphorylated at Ser21.
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.
    Purification
    Affinity chromatography
    Purity
    > 95 % by SDS-PAGE
  • Application Notes
    Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunoprecipitation: 1/50 - 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1,0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    GSK3 alpha (GSK3a)
    Alternative Name
    GSK3 alpha (GSK3a Antibody Abstract)
    Synonyms
    2700086H06Rik, GSK3A, GSK-3[a], GSK-3[b], GSK3, gsk3a, xgsk3, glycogen synthase kinase 3 alpha, glycogen synthase kinase 3 alpha b, glycogen synthase kinase 3 alpha S homeolog, GSK3A, Gsk3a, gsk3a, gsk3ab, gsk3a.S, Bm1_31380
    Background
    Glycogen synthase kinase-3 α and β (GSK-3alpha, beta) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3beta activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFalpha in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3alpha and GSK-3beta induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase)-protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3alpha or Ser 9 of GSK-3beta and inactivates both forms.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha
    Molecular Weight
    approx. 46,51 kDa
    Gene ID
    2931
    NCBI Accession
    NP_063937
    UniProt
    P49840
    Pathways
    PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling
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