CDC25A antibody

Catalog No. ABIN498999

Product Details anti-CDC25A Antibody

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of Cdc25A protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: CDC25A (CDC25A Products)

Synonyms: cdc25a2 antibody, cdc25A antibody, sb:cb317 antibody, CDC25A2 antibody, D9Ertd393e antibody, cell division cycle 25A antibody, cell division cycle 25B antibody, cell division cycle 25A S homeolog antibody, CDC25A antibody, cdc25a antibody, cdc25b antibody, Cdc25a antibody, cdc25a.S antibody

Background: The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40 % identity in their most conserved carboxy terminal sequences.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1

Molecular Weight: approx. 59 kDa

Gene ID: 993

NCBI Accession: NP_001780

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase