POLR2J antibody

Catalog No. ABIN499064

The Rabbit Polyclonal anti-POLR2J antibody is suitable to detect POLR2J in samples from Human and Mouse. It has been validated for WB.

Quick Overview for POLR2J antibody (ABIN499064)

Target: POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2J antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR2J Antibody

Specificity: This antibody detects endogenous levels of POLR2J1 protein. (region surrounding Asp24)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for POLR2J

Target: POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))

Alternative Name: POLR2J

Background: Four independent genes encoding various variants of the hRPB11 subunit of Homo sapiens RNA polymerase II were revealed in human chromosome 7. Three genes (POLR2 J1, POLR2 J2, and POLR2 J3) form a cluster of total length of 214 530 bp in the genetic locus 7q22.1 on the long arm of chromosome 7 (contig NT_007933). The fourth gene (POLR2 J4, 31 040 bp) was localized in the cytogenetic locus 7p13 of the short arm of chromosome 7 (contig NT_007819). An analysis enabled us to refine dissimilar experimental data on the mapping of the hRPB11 subunit gene on chromosome 7. In particular, the presence of three sites of its localization according to data on hybridization with fluorescent-labeled probes (the FISH method) was explained. It was established that, upon the expression of the four human POLR2 J genes, at least 14 types of mature mRNAs encoding somewhat differing hRPB11 isoforms can be synthesized. Eleven of these mRNAs were revealed (as full-length copies or clearly identifiable fragments) in the available databases of expressed sequence tags and cDNAs. The most probable scheme of origination of the multiple genes of the POLR2 J family as a result of three consecutive segmented duplications increasing in size was proposed and substantiated. On the basis of the scheme, some assumptions on the pathways of evolution of separate human genes and the mechanisms of generation of protein diversity in higher eukaryotes were made.Synonyms: DNA-directed RNA polymerase II subunit J-1, DNA-directed RNA polymerase II subunit RPB11-a, POLR2J1, RNA polymerase II 13.3 kDa subunit, RNA polymerase II subunit B11-a, RPB11a

Molecular Weight: approx. 13 kDa

Gene ID: 5439

NCBI Accession: NP_006225

UniProt: P52435

Pathways: Regulatory RNA Pathways