CST8 antibody (Internal Region)

Catalog No. ABIN499119

This anti-CST8 antibody is a Goat Polyclonal antibody detecting CST8 in WB and EIA. Suitable for Human.

Quick Overview for CST8 antibody (Internal Region) (ABIN499119)

Target: CST8 (Cystatin 8 (CST8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CST8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CST8 Antibody

Binding Specificity: AA 100-112, Internal Region

Sequence: STNEICAIQE NSK

Specificity: This antibody recognizes Cystatin 8 (aa 100-112)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_005483.1.

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/16000. Western blot: 1-3 μg/mL. In transfected HEK293 transiently expressing Human CST8 (MYCand DYKDDDDK tagged), a band of approx.23 kDa is observed. No bands are observed inmock-transfected HEK293 and the same band is observed using anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CST8

Target: CST8 (Cystatin 8 (CST8))

Alternative Name: Cystatin 8

Background: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. CST8 performs a specialized role during sperm development and maturation.Synonyms: CRES, CST8, Cystatin-related epididymal spermatogenic protein

Gene ID: 10047

NCBI Accession: NP_005483

UniProt: O60676