FANCL antibody (Internal Region)

Catalog No. ABIN499127

The Goat Polyclonal anti-FANCL antibody is suitable to detect FANCL in samples from Human, Mouse, Rat, Dog and Cow. It has been validated for WB and EIA.

Quick Overview for FANCL antibody (Internal Region) (ABIN499127)

Target: FANCL (Fanconi Anemia, Complementation Group L (FANCL))

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This FANCL antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-FANCL Antibody

Binding Specificity: Internal Region

Sequence: C-QNLKDVLEID FP

Specificity: This antibody is expected to recognize both isoforms (NP_001108108.1, NP_060532.2).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to Human FANCL (NP_001108108.1 and NP_060532.2). Genename: FANCL

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 1-3 μg/mL. Detects a band of approx. 45 kDa in Human Bone Marrow lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FANCL

Target: FANCL (Fanconi Anemia, Complementation Group L (FANCL))

Alternative Name: FANCL

Background: FANCL is a ubiquitin ligase protein that mediates ubiquitination of FANCD2, a key step in the DNA damage pathway.Synonyms: E3 ubiquitin-protein ligase FANCL, FAAP43, Fanconi anemia group L protein, Fanconi anemia-associated polypeptide of 43 kDa, PHF9

Molecular Weight: 43.4kDa (NP_001108108.1).

Gene ID: 55120

NCBI Accession: NP_001108108

UniProt: Q9NW38

Pathways: DNA Damage Repair