ARHGDIG antibody (C-Term)

Catalog No. ABIN499143

The Goat Polyclonal anti-ARHGDIG antibody (ABIN499143) specifically detects ARHGDIG in WB and EIA. The antibody is reactive with Human, Mouse, Rat, Dog and Cow samples.

Quick Overview for ARHGDIG antibody (C-Term) (ABIN499143)

Target: ARHGDIG (rho GDP Dissociation Inhibitor (GDI) gamma (ARHGDIG))

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ARHGDIG antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ARHGDIG Antibody

Binding Specificity: C-Term

Sequence: C-EAVPEYRAPG RK

Specificity: Recognizes ARHGDIG (C-term).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_001167.2. Genename: ARHGDIG

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.5-2 μg/mL. This antibody detects a band of approx. 25 kDa in lysates of cell lineHeLa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ARHGDIG

Target: ARHGDIG (rho GDP Dissociation Inhibitor (GDI) gamma (ARHGDIG))

Alternative Name: ARHGDIG

Background: ARHGDIG (Rho GDP dissociation inhibitor (GDI) gamma) inhibits the GDP/GTP exchange reaction of RhoB. It interacts specifically with the GDP- and GTP-bound forms of post-translationally processed RhoB and RhoG proteins, both of which show a growth-regulated expression in mammalian cells. ARHGDIG stimulates the release of the GDP-bound but not the GTP-bound RhoB protein.Synonyms: Rho GDI 3, Rho GDP-dissociation inhibitor 3, Rho-GDI gamma, RhoGDI3

Molecular Weight: 25.1kDa (NP_001167.2).

Gene ID: 398

NCBI Accession: NP_001167

UniProt: Q99819